ELISA Test (Enzyme-linked immunoassay)
The enzyme-linked immunoassay (ELISA) is used to identify antibodies in the blood produced due to a harmful substance that has entered the body. This test is widely used to detect blood-borne viruses like HIV, HBV, HCV, and HTLV.
Four types of ELISA- Direct ELISA, Indirect ELISA, Sandwich ELISA, and Competitive ELISA.
Cost in India
Test Type | Blood Tests |
---|---|
Preparation | There is no special preparation for this test. The patient can eat and drink anything before the test. |
Report | Within a day. |
ELISA test costs in Hyderabad | Rs. 500 to Rs.1500 approximately. |
ELISA test costs in Vizag | Rs. 500 to Rs.1500 approximately. |
ELISA test costs in Nashik | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Aurangabad | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Nellore | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Chandanagar | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Srikakulam | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Sangamner | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Kurnool | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Kakinada | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Karimnagar | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Zaheerabad | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Sangareddy | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Nizamabad | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Mumbai | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Begumpet | Rs. 500 to Rs.1500 approximately |
ELISA test costs in Vizianagram | Rs. 500 to Rs.1500 approximately |

Book a ELISA test at Medicover Hospitals. Call us at 040-68334455
Findings of ELISA test
The ELISA test helps identify the situations that lead the immune system to produce antibodies, which the swab tests cannot easily identify.
It helps find out about the viruses like HIV, HBV, HCV, and HTLV in the blood that lead the body to produce antibodies.
You must visit a doctor for abnormal findings and get a complete diagnosis and treatment.
Frequently Asked Questions
1. Can ELISA identify multiple antibodies?
No, each ELISA can detect a specific antigen.
2. What does it mean by heterophilic antibodies?
The plasma of the human body where the heterophilic antibodies are found is capable of binding to both the capture and detection antibodies used in capture ELISA by cross-linking with the assay antibodies and resulting in false-positive signals.
3. What is the normal value for ELISA test?
Typical ELISA test detection ranges from 0.1 to 1 fmole or 0.01ng to 0.1ng.
4. When is an Elisa test recommended?
This test is recommended if you are exposed to HIV as well as to diagnose other infectious conditions.
5. Which enzyme is used in the ELISA test?
Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) are commonly used enzymes.
6. When is the ELISA test accurate?
The test can be accurate by 99.9% when used in combination with the confirmatory Western blot test.
7. What is the principle of the ELISA test?
Specific antibodies bind to the target antigen by detecting the presence of antigens, and to get the correct value, the plate must be coated with the antibodies with high affinity.
8. When should the ELISA test be done?
When a person has some infectious conditions, the test determines if the patient has antibodies developed due to any infection.
9. Why an Elisa test is so sensitive?
The ELISA test is sensitive due to the binding characteristics of the antibodies.
10. How much blood is needed for an Elisa test?
It requires a small draw of blood, around 4 milliliters.